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Small and microRNA Sequencing

Small and microRNA Sequencing
 
 
Unlock the regulatory landscape of non-coding RNA
Our small RNA sequencing service enables you to investigate post-transcriptional regulation, discover novel small RNAs, and identify condition-specific biomarkers. Whether you're working in basic research or drug development, this service adds a vital layer of insight beyond traditional RNA-seq.

What You Can Achieve

  • Reveal the regulatory impact of miRNA, piRNA, and other small RNAs
  • Identify novel small RNA species and expression profiles
  • Detect biomarkers under specific physiological or disease conditions
  • Uncover gene regulation in functional or therapeutic studies
  • Support drug discovery and target validation
  • Complement your omics data with non-coding RNA insights

Before You Start

We support you from experimental design to analysis. Key questions to clarify:

  • What small RNA types and size ranges are expected (known vs. novel)?
  • Which sample types are used (e.g., serum, cells, tissues, organisms)?
  • What sequencing depth and number of replicates are needed for reliable insights?
  • Is your organism well annotated, or will de novo discovery be needed?
  • Are reference miRNA/piRNA databases available and applicable?

Modular Workflow

Outsource the entire workflow—or select specific modules. Our process is designed for flexibility. Typical workflow steps include:

Small and microRNA Sequencing

Bioinformatic Analysis

Our standard pipeline delivers both depth and clarity, including:

  • Data QC & filtering: Raw data quality control, read trimming, and length filtering
  • Mapping & quantification: Alignment to reference genomes and RNA databases
  • Classification: Categorization of known small RNA classes (e.g., miRNA, piRNA, snoRNA)
  • Novel discovery: Prediction of new small RNA candidates
  • Expression analysis: Differential expression across conditions or treatments
  • Target gene annotation: Prediction and functional analysis (GO and KEGG enrichment)
  • Motif analysis: Detection of sequence motifs in novel miRNAs

You receive raw files, normalized data, and publication-ready summaries.

Turnaround Time

  • 25 working days for library preparation and sequencing
  • +10 working days for standard bioinformatics
  • Express service available upon request

Sample Requirements

  • Buffer recommendation: 10 mM Tris-HCl (pH 7.0)
  • Important: Avoid any buffers containing EDTA >1mM
  • RNA quantification: Use fluorometric methods (e.g., PicoGreen®, Qubit®)

Minimum Input for Illumina Sequencing:

Library Type Amount (µg) Concentration (ng/µl)
Small RNA / miRNA (total RNA) >1.0 >20
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